The Potential of Tinospora cordifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa

Mitigation and control of bacterial corrosion must have been prioritized for failure anticipation by associated with microorganisms. Using synthetic inhibitor material protection from problems because it is not eco-friendly. This work conducted preliminary studies on T. cordifolia stem extracts at various methanol ratios against P. aeruginosa biofilm as a new eco-friendly inhibitor. The was extracted the maceration method different ratio solvent (100, 75 50 %). activity assessed using dilution (MTT assay) to determine minimum inhibitory concentration (MIC). total phenolic flavonoid contents were determined Follin-Ciocalteu aluminum chloride (AlCl3) colorimetric, respectively. Meanwhile, structure active compounds in extract identified liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). yields are 9.45, 7.56 8.40 % 100, ratios, content (TPC) crude 11.20, 9.46 6.56 ratios. (TFC) obtained about 5.25, 0.64 0.33 concentrations, has antibacterial all concentrations. (MIC) found 4096 µg/mL. Methanol most %, followed 100 %. structures compound methanolic group, alkaloid derivatives (Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, Yuanhunine). HIGHLIGHTS Tinospora potential marine environment µg/mL 50:50 (v/v) water Calopiptin, Yuanhunine GRAPHICAL ABSTRACT